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If need to sedate your fish, Jambu (Spilanthes acmella) or Lippia alba may be an option

Posted: 22 Apr 2016, 23:26
by bekateen
Barbasa, LAL, Stringhetta, GR, Garcia, LO, Figueiredo, MRC, & Sampaio, LA. 2016. Jambu, Spilanthes acmella as a novel anaesthetic for juvenile tambaqui, Colossoma macropomum: Secondary stress responses during recovery. Aquaculture, 456, pp. 70–75. doi:10.1016/j.aquaculture.2016.01.026
Barbasa et al. wrote:ABSTRACT

The aim of this study was to evaluate the efficacy of the waxy extract of jambu flowers, Spilanthes acmella as an anaesthetic for fish, using juvenile tambaqui as a model. The times to induction and recovery after short-term anaesthesia were evaluated by concentration-response trials and secondary stress responses. Juveniles were placed in aquaria containing five different concentrations of jambu extract (5, 10, 15, 20, 25 mg L−1) and the times of anaesthetic induction and recovery were determined. Sham control fish and fish exposed to ethanol-added water were used as controls. The secondary stress responses of fish following anaesthesia with jambu extract (20 mg L− 1) were investigated through an assessment of whole blood variables: glucose, ions (Na+, K+, and Ca++), osmolality, haematocrit (Htc), haemoglobin (Hb), partial pressures of CO2 (pCO2) and O2 (pO2), bicarbonate concentration (HCO3−), and pH. Deep anaesthesia was observed at all concentrations tested in this study. The use of 20 mg L− 1 of this extract is recommended for rapid induction (< 3 min) and uneventful recovery (< 5 min) from deep anaesthesia; while the concentration of 2 mg L− 1 is sufficient to promote sedation. Only transient changes in secondary stress responses were observed in tambaqui during recovery, with most parameters returning to initial values within 48 h post-anaesthesia. Therefore, the extract of jambu flowers may be considered an efficient anaesthetic for tambaqui and other fish species.
  • Highlights:
    • This is the first report of jambu extract being used as an anaesthetic for fish.
    • All concentrations tested (5–25 mg L−1) induced deep anaesthesia and uneventful recovery in juvenile tambaqui.
    • Only transient physiological changes were observed in blood during recovery.
    Statement of relevance: This is the first study using extract of Jambu, Spilanthes acmella as an anaesthetic for aquatic organisms.

    Keywords: Sedation; Brazil cress; Plant extract; Spilanthol; Blood parameters

    KeyWords Plus: Salmon ; ; ; tricaine methanesulfonate (MS-222); Ocimium gratissimum; essential oils; ; Lippia alba; clove oil; euglenol

Personally, I rely on benzocaine. But if you don't have access to benzocaine, I know some of you have used plant extracts before; here's one more potential "tool" for your "toolbox."

Re: If need to sedate your fish, Jambu (Spilanthes acmella) or Lippia alba may be an option

Posted: 22 Apr 2016, 23:48
by bekateen
Or maybe you can use Lippia alba extract:

Hohlenwerger, JC, Copatti, CE, Sena, AC, Couto, RD, Baldisserotto, B, Heinzmann, BM, Caron, BO, & Schmidt, D. 2016. Could the essential oil of Lippia alba provide a readily available and cost-effective anaesthetic for Nile tilapia (Oreochromis niloticus)? Marine and Freshwater Behaviour and Physiology, 49(2), pp. 119-126. DOI: 10.1080/10236244.2015.1123869
Hohlenwerger et al. wrote:ABSTRACT

We report the optimal concentration of the essential oil of Lippia alba (EOLA) for the induction and recovery of anesthesia in Nile tilapia () during handling. Groups of 10 juveniles were separately subjected to a concentration of EOLA: 0 (control group), 10, 20, 50, 100, 200, 300, or 500 mu L L-1. The plasma cortisol levels were significantly decreased in anesthetized fish 4 h after stress of handling. The plasma glucose levels were significantly increased 1 and 4 h after stress of handling in fish exposed to EOLA. Sensory analysis showed that tasters did not detect differences through a blind test in fillet taste and odor between exposed and non-exposed fish. The ideal concentration to promote the best anesthesia and recovery times and to reduce plasma cortisol 4 h after handling is 500 mu L L-1.