More info on Genetic diversity of Indonesian indigenous catfish

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bekateen
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More info on Genetic diversity of Indonesian indigenous catfish

Post by bekateen »

Related study:
bekateen wrote: Sat Jan 23, 2021 4:46 am Budiariati, V., Susmiati, T., Munawaroh, S., Putri, R. C. A., & Widayanti, R. W. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas Journal of Biological Diversity, 22(2).
https://www.smujo.id/biodiv/article/view/7400
New study:
Widayanti, R., Kusumaastuti, K.A., Novi, J.M., Adani, F.K., Gultom, C.R.P, Prastiti, A.D., Nugroho, H.A., & S. Pakpahan. (2021). Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene. Veterinary World, 14(3): 751-757. DOI: HTTP://www.doi.org/10.14202/vetworld.2021.751-757

http://www.veterinaryworld.org/Vol.14/M ... 21/27.html
PDF: http://www.veterinaryworld.org/Vol.14/March-2021/27.pdf
Abstract
Background and Aim: Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene.
Materials and Methods: In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences were analyzed using MEGA X to determine genetic variation and phylogenetic relationships.
Results: In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08.
Conclusion: Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS, BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.
  • Keywords: 12S ribosomal RNA gene, baung fish, Hemibagrus nemurus, Indonesian catfish, phylogenetic.
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Re: More info on Genetic diversity of Indonesian indigenous catfish

Post by Barberry »

Wow! I could never think about how diverse is this specious! I conducted a study about bees in my region and was also surprised about the results! I find especially interesting the way to get the genomes from the epaxial and hepaxial muscle samples. Never thought about that. Where have you found the study that you posted here? I'd like to read more of those articles. Btw. any of you could share some websites where the subscription is not too expensive? I had some subscriptions on Emerald and Science Direct, but I found them a bit too expensive for my needs. And I feel nervous when I have to use google scholar.
Last edited by Barberry on Thu Jun 03, 2021 9:30 am, edited 1 time in total.
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bekateen
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Re: More info on Genetic diversity of Indonesian indigenous catfish

Post by bekateen »

I use Google scholar mostly.

Cheers, Eric
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