A not-yet-peer-reviewed paper on Hypancistrus zebra

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A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by bekateen »

Torres de Barros, Fábio José and Lobato, Isadora Ribeiro da Silva and Nascimento, Thaís Pereira and Moura, Mayllon and Torres, Rayane Arcanjo and Pereira, Tatiana da Silva and Sousa, Leandro Melo de. (2024). Effect of Stocking Density on Growth of the Endangered Fish Hypancistrus Zebra (Siluriformes: Loricariidae). Available at SSRN: https://ssrn.com/abstract=4699861 or http://dx.doi.org/10.2139/ssrn.4699861

Torres de Barros, Fábio José and Lobato, Isadora Ribeiro da Silva and Nascimento, Thaís Pereira and Moura, Mayllon and Torres, Rayane Arcanjo and Pereira, Tatiana da Silva and Sousa, Leandro Melo de. (2024). Efeito Da Densidade De Estocagem No Crescimento Do Peixe Ameaçado De Extinção Hypancistrus Zebra (Siluriformes: Loricariidae). Available at SSRN: https://ssrn.com/abstract=4699861 or http://dx.doi.org/10.2139/ssrn.4699861
Abstract
Stocking density has a significant influence on animal homeostasis, being a crucial factor in the management of organisms in captivity and an important role in the ex-situ conservation of threatened species. is a fish species endemic to the Xingu River, Brazilian Amazon, considered Critically Endangered since the early 2000s due to fishing pressure associated with the aquarium trade market and, more recently, changes in habitat caused by the Belo Monte Hydroelectric Plant. The implementation of adequate management in captivity is extremely important for its conservation, especially in improving ex-situ breeding techniques. To deepen knowledge in this area, this study aimed to evaluate the growth of juvenile H. zebra in aquariums with three different levels of stocking density. Juveniles were distributed into three treatments, each with three replications. Biometric measurements were performed using ImageJ software, allowing individual lengths to be measured. The relationship between stocking density and growth was analyzed using a correlation matrix adapted to the Pearson method, while the growth pattern was examined using polynomial regressions. Comparison of growth averages between different densities was performed using Student's t test for independent samples. There was no correlation between biomass and aquarium volume. The highest correlation coefficients were found between growth rates and densities related to the number of individuals per shelter area. The mortality rate was directly related to the initial number of fish in the aquariums, with larger populations showing higher mortality rates. The treatment with lower storage density showed a higher growth rate, while the treatment with higher density showed a lower growth rate, with a difference of 23.38% between them.
Abstract
A densidade de estocagem tem uma influência significativa na homeostase dos animais, sendo um fator crucial no manejo de organismos em cativeiro e um papel importante na conservação ex-situ de espécies ameaçadas. é uma espécie de peixe endêmica do Rio Xingu, Amazônia brasileira, considerada Criticamente Ameaçada desde o início dos anos 2000 devido à pressão pesqueira associada ao mercado de comércio de aquários e, mais recentemente, às mudanças de habitat causadas pela Usina Hidrelétrica de Belo Monte. A implementação de um manejo adequado em cativeiro é de extrema importância para a sua conservação, principalmente no aprimoramento das técnicas de criação ex-situ . Para aprofundar o conhecimento nesta área, este estudo teve como objetivo avaliar o crescimento de juvenis de H. zebra em aquários com três diferentes níveis de densidade de estocagem. Os juvenis foram distribuídos em três tratamentos, cada um com três repetições. As medidas biométricas foram realizadas utilizando o software ImageJ, permitindo a medição de comprimentos individuais. A relação entre densidade de estocagem e crescimento foi analisada através de uma matriz de correlação adaptada ao método de Pearson, enquanto o padrão de crescimento foi examinado através de regressões polinomiais. A comparação das médias de crescimento entre as diferentes densidades foi realizada através do teste t de Student para amostras independentes. Não houve correlação entre biomassa e volume do aquário. Os maiores coeficientes de correlação foram encontrados entre taxas de crescimento e densidades relacionadas ao número de indivíduos por área de abrigo. A taxa de mortalidade esteve diretamente relacionada ao número inicial de peixes nos aquários, com populações maiores apresentando taxas de mortalidade mais elevadas. O tratamento com menor densidade de estocagem apresentou maior taxa de crescimento, enquanto o tratamento com maior densidade apresentou menor taxa de crescimento, com diferença de 23,38% entre eles.
Keywords: zebra pleco, L46, conservation, ornamental fish, Xingu River.
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by dw1305 »

Hi all,
Juveniles were distributed into three treatments, each with three replications
Three, it is obviously the magic number. Do we know what the stocking densities were? Without wishing to prejudge the issue I'm not sure that is a paper that is going to tell us (or anyone else) anything useful.

cheers Darrel
Last edited by dw1305 on 04 Feb 2024, 11:34, edited 1 time in total.
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by bekateen »

I haven't read the full paper, but I believe there was a link to a pdf.

Cheers, Eric
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by helepolis »

dw1305 wrote: 30 Jan 2024, 13:49 Hi all,
Juveniles were distributed into three treatments, each with three replications
Three, it is obviously the magic number. Do we know what the stocking densities were? Without wishing to prejudge the issue I'm not sure that is a paper that is going to us (or anyone else) anything useful.

cheers Darrel
If I'm reading right on page 15, starting on line 440 of the pdf it looks like the stocking densities were:
100l tank #1 initially 3, tank #2 initially 7, and tank #3 initially 13.
50l tank #1 initially 13, tank #2 initially 8, and tank #3 initially 5.
25l tank #1 initially 11, tank #2 initially 8, and tank #3 initially 9.

All stock is from natural spawns, hence random numbers.

They also had a pretty high amount of deaths, as someone struggling with fry I feel a bit better that I'm not doing as bad as I thought haha.

It seems their conclusion was the tank volume didn't matter so much(all tanks were connected to a central filter sump as well as hmf in each tank) but the available shelter area did. A stocking density of 2.58 x 10-4 individuals/cm² of shelter was found to be ideal with on average of 766 days to reach 5.5cm.
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by dw1305 »

Hi all,
helepolis wrote: 30 Jan 2024, 21:13 ..... If I'm reading right on page 15, starting on line 440 of the pdf it looks like the stocking densities were:
100l tank #1 initially 3, tank #2 initially 7, and tank #3 initially 13.
50l tank #1 initially 13, tank #2 initially 8, and tank #3 initially 5.
25l tank #1 initially 11, tank #2 initially 8, and tank #3 initially 9.

All stock is from natural spawns, hence random numbers.

They also had a pretty high amount of deaths, as someone struggling with fry I feel a bit better that I'm not doing as bad as I thought haha.

It seems their conclusion was the tank volume didn't matter so much(all tanks were connected to a central filter sump as well as hmf in each tank) but the available shelter area did. A stocking density of 2.58 x 10-4 individuals/cm² of shelter was found to be ideal with on average of 766 days to reach 5.5cm.
Unfortunately some scientific journal will publish that.

As I was reading it I had this vision of a student who came to see once and told me that they had a "perfect correlation" from their experiment, and when I started looking at their data I realised that they only had two data points. ..........

cheers Darrel
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by Shane »

Not to make light of the paper but the same outcome has been realized dozens of times in published aquaculture research. Crowding is the main factor that limits fry growth. This holds true in better controlled experiments than the above.

Raising a bunch of Cory fry in different tanks at different stocking levels and measuring growth rates makes a good High School science fair project.
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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by bekateen »

Shane wrote: 02 Feb 2024, 12:36Raising a bunch of Cory fry in different tanks at different stocking levels and measuring growth rates makes a good High School science fair project.
-Shane
One of my daughters attempted a stocking level experiment with guppies for her junior high school science fair project. Even though she used containers with cycled filters, she had the stocking densities so high in some tanks that almost everything died. She was traumatized and said she never wanted to have fish again. #-O

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Re: A not-yet-peer-reviewed paper on Hypancistrus zebra

Post by Shane »

🙁
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